MORFOLOGI DAN KARAKTERISASI ISOLAT AKTINOBAKTERIA PADA
TANAMAN BAWANG MERAH (Allium ascalonicum L.) DI KABUPATEN SOLOK
Sixsri Selviana1, Yulmira Yanti1, Yenny Liswarni1
Program Studi Proteksi Tanaman, Fakultas Pertanian, Universitas Andalas, Padang, Sumatera Barat, Indonesia
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Diterima / Disetujui
ABSTRACT
Actinobacteria are a group of Gram-positive bacteria that are rod-shaped, facultatively anaerobic and have branched mycelia resembling a fungus. Actinobacteria are one of the largest bacterial phyla in colonized terrestrial and aquatic ecosystems, can live in extreme environments, contain secondary metabolites that are useful for biotechnology and agriculture. Although actinobacteria are unicellular like bacteria in general, the walls of actinobacteria are different from ordinary bacteria, where actinobacteria have an uninsulated and slender mycelium. This study aims to determine the morphology and characterization of actinobacteria isolates on shallot plants. This study used a randomly selected method. Samples were taken in Solok district, 3 Nagari were selected from shallot plantations. Actinobacteria isolation as much as 1 gram of soil sample was put into a test tube containing 9 ml of sterile distilled water, homogenized with a vortex, then diluted to 10-7. The suspension from the 10 -6 and 10-7 dilutions was taken 1 ml and then put in a test tube that already contained each ISP2 medium, and the SCA media that had been thawed were then homogenized with a vortex. The suspension was put into a petri dish and incubated for 4x24 hours. The results showed that from the isolation results obtained 11 actinobacteria isolates with diverse morphology and characterization, this can be seen from the various forms and colors of actinobacteria. The morphology and characteristics of actinobacteria that have different spiral-shaped spore chains and some do not have spirals and the hyphae of actinobacteria vary.
Key words : Actinobacteria, shallots, characterization, morphology.
